Hoefer PR150 - Manual (Page 4)

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The Hoefer ® PR150 has been designed specifi-
cally to preserve this correspondence while mini-
mizing the volume of reaction mixtures needed
for individual assays. Two sturdy acrylic plates,
each 1 cm thick and 13 × 13 cm square, clamp
an 8.8 × 8.8 cm transfer membrane between two
silicone gaskets. Ten parallel troughs cut into
one side of the upper plate become individual
incubation chambers when the unit is assembled.
The gaskets ensure that the liquid samples and
probes do not leak into the adjacent chambers.
All incubation steps may be carried out on the
intact membrane without taking the unit apart.
Since the membrane is firmly held between the
two plates, it cannot float to the surface of the
reaction mixture or trap bubbles underneath.
Gentle rocking of the unit fully exposes the
membrane surface to small volumes of reagents,
thus preventing artifacts caused by incomplete
exposure or mixing. Since reaction chambers are
fully enclosed, hazardous materials may be safely
contained.
The PR150 has two principal applications:
1. Up to ten probes or antisera may be tested
against a single sample of protein or nucleic
acids. The sample is loaded onto a slab gel in
a single, full-width well. When clamped in the
PR150, ten different probes or antisera may
be tested against the same separated materials.
2. It is appropriate for efficient use of precious
or small volumes of probes or antisera that
need to be tested against several samples.