Hoefer PR150 - Manual (Page 3)

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Introduction
Transfer of proteins or nucleic acids, fraction-
ated by gel electrophoresis, to an immobilizing
membrane surface increases the sensitivity of
a wide range of detection methods, reduces
time of analysis and makes sequential probing
possible. In particular it has made possible use
of immunological procedures that are not practi-
cal to carry out in a gel. To assay material on a
sheet of membrane with different probes simul-
taneously commonly requires first cutting the
membrane into a number of parallel strips. This
procedure however destroys the correspondence
between different lanes or positions on a gel.
Fig 1. Components of the PR150.
Pressure gasket
sample gasket
gasket
seating lip
sealing ridge
sample
chamber
Pressure plate Sample plate
gasket
seating lip