Hoefer TE70X - Manual (Page 14)

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Complete the stack
a. Place the pre-wet membrane onto the stack of
blotting paper.
b. Place the gel on the membrane.
Note: Proteins bind to the membrane as soon as
contact occurs, so it is important to place the gel
correctly on the first try.
c. Cover the gel with three layers of buffer saturated
blotting paper.
Important! Stack each layer with
care, with edges parallel. As each
layer is added, remove all air
pockets by rolling a clean pipet
from the center to the edges. Add
a few drops of buffer to trouble
areas to help remove air pockets.
Note: If not using Hoefer blotting
paper, additional pieces may be
required to achieve desired results.
Mask (opening approx. 2 mm smaller
than the gel on all sides). Optional.
Blotting paper
(2–3 sheets)
Blotting paper
(2– 3 sheets)
Gel
Membrane
These stack
components are
the same size
as the gel or
slightly smaller
Fig 2. Transfer stack for a
single gel.