Hoefer TE70X - Manual (Page 12)

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2. Operating instructions
To transfer proteins, prepare the unit, assemble
the stack, and connect to a power supply if
necessary. Then run the transfer for the required
amount of time. Each step is described below.
1
Prepare the unit by rinsing the electrodes with
distilled water.
2
Prepare the gel
Cut away the wells and/or stacking gel section.
Equilibrate the gel in transfer buffer if required.
3
Prepare the transfer stack
Cut the blot paper and transfer membrane to the
same size as the gel. Stack the layers carefully so
the edges align. If for some reason the membrane
needs to be larger than the gel, use a mylar mask
(see optional step below) to ensure the current does
not bypass the gel.
Optional: Cut a mask to the proper size
Measure the gel. Cut an opening centered in the solid
mask to a size roughly 2 mm smaller than the gel.
Take care with sharp blades when cutting the masks.
Place the mask in the base of the unit, centering
the opening. Note: Take care to place the
gel correctly on the first try
because proteins begin to transfer
immediately; once transfer has
begun, moving the gel will distort
results or cause “shadow” bands
on the blot.