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BUCHI Cartridge C-670 User Manual

Page 16

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BÜCHI Cartridger

TM

C-670

14

Figure 5.7: Tools

5.2.7 Inserting the second (upper) frit

Before inserting the second frit you must first use the appro-
priate tool (knife

ቢ or ባ) in the lid to free the column start of

gel. The scraper knives have a stop that determines the depth.
Now insert the appropriate frit with 12 mm or 40 mm into the
insertion tool and insert the second frit.

ቢ Knife Ø 12 mm
ባ Knife Ø 40 mm
ቤ Punch Ø 40 mm
ብ Punch Ø 12 mm
ቦ Brush set Ø 40 mm complete
ቧ Stop sleeve

5.2.8 Removing the packed cartridge

Close the vacuum valve and unscrew the cartridge from the
cartridge holder. Finished. You can now use the cartridge for
chromatography.
Avoid jarring or blows. These can damage the column bed,
which unavoidably leads to worse separation performance.

5.3

Storing the cartridges

Büchi recommends packing the cartridges immediately prior
to use. If you intend to store supplies of filled cartridges, be
sure that the cartridge ends are properly sealed and that they
are stored protected from jarring and vibration. Most gels are
hygroscopic and have a bonding affinity with volatile chemicals.
Both effects have a negative influence on the separation
performance.

5.4

Changing the stationary phase

When changing the stationary phase it is particularly important
to thoroughly clean the glass vessel and the lid. This is best
undertaken with a vacuum cleaner and a dry, lint-free cloth.
Never use solvents or water!

5 Operation