BUCHI Fraction Collector C-660 - Manual (Page 25)

6 Operation
25 C-660 Operation Manual, Version F
6 .3 .4 Interface RS 232
The interface works with 9600 Baud, straight parity, 8 data bits and 1 stop bit.
Output is provided via a PC or a printer. An example is shown in Figure 5.8.
The header of the table is always written in English and appears when a program is started (transition
from rest status into collecting status). Data lines are then written in an interval of one second until the
program is finished or the rest status has been reached again.
Time (s) Flow A
(ml/min)
Flow B
(ml/min)
Rack
R4
X Y Tube Waste Det 1 (%)
100% = 1 V
Det 1 (%)
100% = 1 V
Pressure
(bar)
1
2
3
4
5
6 100
100
100
100
100
100 0
0
0
0
0
0 1
1
1
1
1
1 760
760
760
2260
2260
760 470
470
470
1560
1560
1560 0
1
2
3
4
5 X 123
121
123
130
131
133 8
8
8
7
6
7 0
0.5
1.1
1.2
1.2
1.2
The „Time“ column displays the time passed since the beginning of the program in seconds. The
range extends from 0 to 999999s (app. 15 days). It subsequently starts again at zero.
The tube number („Tube“) is given as 0 and the character „x“ is written in the „Waste“ column in the
Wait, Pause and Error states. While the arm is moving from one position to the next, the new tube
number and tube position are entered and the „Waste“ column is not marked.
The „Flow A“ and „Flow B“ columns indicate the flow in milliliters per minute with two places after the
decimal. The resolution is thus 10 µ/min.
If work is carried out without a BUCHI pump, the low entry is always 0.
The position of the collector arm is given in columns „X“ and „Y“ in 0.1 mm steps.
The flow rate in the protocol corresponds to the value displayed on the pump. However, the rotational
speed of the pump can deviate by up to 20 % due to the calibration. The actual rotational speed of
the pump is not given in the protocol.
6 .3 .5 Peak detection
General:
The fraction collector is normally set to the detector signal of 1 V end-scale deflection.
A calm detector signal is the prerequisite for a problem-free peak collection function. Most detectors
have adjustable time constants, which serve to smoothen the signal.
Function:
The integrated peak detector enables an automatic fraction change on the basis of the minima and
maxima of the peak. The following definitions are necessary for the configuration of the fraction
collector: