ANKOM 2000 Series - Manual (Page 40)

Operator’s Manual
pg. 40
Rev E 1/14/15
Calculations
% NDF (as-received basis) = 100 x (W
3
– (W
1
x C
1
))
W
2
Where: W
1
= Bag tare weight
W
2
= Sample weight
W
3
= Dried weight of bag with fiber
after extraction process
C
1
= Blank bag correction (running
average of final oven-dried
weight divided by original blank
bag weight)
Numbered Notes
1. A running average blank bag correction factor (C
1
) should be
used in the calculation of fiber. The inclusion of at least one
blank bag in each run is mainly used as an indicator of particle
loss. A C
1
larger than 1.0000 indicates that sample particles were
lost from filter bags and deposited on the blank bag during the
extraction. Any fiber particle loss from the filter bags will
generate erroneous results. If particle loss is observed then the
grinding method needs to be evaluated.
NDF Procedure (continued)
10. If you are using Cubetainers for your chemicals, attach the
ND solution hose to the Cubetainer and then to Port A on the
instrument.
11. Open the Vessel Lid and insert the Bag Suspender with bags
into the Vessel and place the Bag Suspender weight on top
of the empty 9
th tray to keep the Bag Suspender submerged.
12. Follow the instructions on the ANKOM 2000 display:
a. Select NDF. (Wait to close the Vessel Lid.)
b. Confirm hot water is on (>70°C).
c. Press START.
d. After the ND solution has been automatically
inserted and agitation begins, manually add 20g of
Na
2
SO
3
and 4.0ml of alpha-amylase.
e. Close the Vessel Lid.
13. Attach the Amylase Dispenser Assembly to Port B on the
instrument. Add 8.0ml of alpha-amylase and enough water
to fill the dispenser. The ANKOM
2000 will automatically add
the amylase solution to the first and second rinse.
14. When the NDF extraction and rinsing procedures are
complete, open the Vessel Lid and remove the filter bags.
Gently press out excess water from the bags. Place bags in a
250ml beaker and add enough acetone to cover bags and
soak for 3-5 minutes.
15. Remove the filter bags from the acetone and place them on a
wire screen to air-dry. Completely dry in an oven at 102 ±
2°C. (In most ovens the filter bags will be completely dry
within 2-4 hours.) NOTE: Do not place bags in the oven
until the acetone has completely evaporated.
16. Remove the filter bags from the oven and immediately place
them directly into a collapsible desiccant pouch and flatten
to remove any air. Cool to ambient temperature and weigh
the filter bags (W
3
). NOTE: Do not use a conventional
countertop or cabinet desiccator.