ANKOM 2000 Series - Manual (Page 21)

Operator’s Manual
Rev E 1/14/15
pg. 21
NDF Analysis Procedure using the ANKOM
2000
Fiber Analyzer
To perform NDF analysis on prepared samples, follow the procedure detailed below.
IMPORTANT

If you are changing from one analytical method to another, flush the system before
running. See the “Flush Procedure” section of this manual for more information.

Amylase tends to be sticky. You should always clean the Amylase Dispenser
Assembly and flush Port B after every NDF procedure unless you are running another
NDF procedure with less than two hours between procedures.
1. Verify that the hot water supply is on and the drain hose is securely positioned in the drain.
2. If you are using Cubetainers for your chemicals, attach the ND solution
hose first to the Cubetainer and then to Port A on the instrument.
IMPORTANT
The solutions are gravity fed into the instrument ports. The bottom of the solution level
must be at least 5” (13cm) above the port. The top of the solution level can be no more
than 20” (50cm) above either port or the solution will overflow into the drain.
3. Open the Vessel Lid.
4. Place the bag suspender with the samples into the Vessel.
5. Place the Bag Suspender Weight onto the Bag Suspender rod to hold the trays in place.
6. Attach the Amylase Dispenser Assembly to Port B. This will be used to
automatically add amylase to the vessel during the rinses.
7. Fill half of the dispenser with water.
8. Add two capfuls (8ml) of amylase to the dispenser.
9. Fill the dispenser with water.
10. Turn the instrument Power Switch to the ON position. The Display will light and allow you to select an
analysis procedure.