ANKOM 2000 Series - Manual (Page 20)

Operator’s Manual
pg. 20
Rev E 1/14/15
5. Place an empty filter bag in the Bag Holder in an open position.
6. Tare the weight of the empty filter bag and the holder together.
7. Add 0.45 – 0.50g of sample to the filter bag. Keep all particles away from the
sealing area of the filter bag.
8. Record the weight of the sample (W
2
) and tare.
9. Seal the filter bag within 4mm of its open end. Keep the sealer arm down for 2 – 3 seconds after the red
sealer light turns off (to cool the seal). The seal can be seen as a solid melted stripe along the top edge of
the filter bag. If the seal is not strong, re-seal the bag.
10. Spread the sample out uniformly within the filter bag by shaking and flicking the bag to eliminate
clumping.
11. Repeat steps 5 – 10 for all filter bags that will be used in the Analyzer. (Up to 24 bags can be processed
during one procedure.)
IMPORTANT
If your samples contain soybean products or >5% fat, before doing the NDF analysis in
the ANKOM
2000 , you will need to do a pre-extraction. For samples containing non-roasted
soybean or >5% fat, follow the pre-extraction steps below:
1. Place the filter bags with sample (up to 23) into a container with a top.
2. Pour enough fresh acetone into the container to cover the bags.
3. Put the top on the container.
4. Shake the container 10 times and allow bags to soak for 10 minutes.
5. Pour out and dispose of the acetone.
6. Execute steps 1 through 5 a total of two times.
7. Place the bags on a wire screen to air-dry.
If your samples contain roasted soybean, follow the pre-extraction steps below:
1. Place the filter bags with sample (up to 23) into a container with a top.
2. Pour enough fresh acetone into the container to cover the bags.
3. Put the top on the container.
4. Shake the container 10 times.
5. Pour out and dispose of the acetone.
6. Pour fresh acetone into the container and allow the samples to soak for twelve
hours.
7. Pour out the acetone.
8. Place the bags on a wire screen to air-dry.
12. Place the filter bags with sample and at least one empty bag (used as a Blank) into
the Bag Suspender trays as shown (maximum of three bags per tray).
13. Stack each tray on the Bag Suspender rod (eight trays in total) with each tray
rotated 120 degrees from the tray below.
IMPORTANT
You must use all eight trays even if they are empty.
14. Add the 9
th
tray to the top of the Bag Suspender rod. This tray contains no filter bags and acts as a cover.
The samples are now ready for the NDF analysis procedure.