Bio-Rad Comparative Proteomics Kit II: Western Blot Module - Manual (Page 3)

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Quick Guide
Q
UICK GUIDE
11. Heat fish samples and actin and myosin
standard to 95°C for 2–5 min.
12. Load your gel:
Lane Volume Sample
1 & 2 empty Empty
3 5 µl Precision Plus Protein
Kaleidoscope
prestained standards
4 5 µl fish sample 1
5 5 µl fish sample 2
6 5 µl fish sample 3
7 5 µl fish sample 4
8 5 µl fish sample 5
9 5 µl actin and mysin standard
(AM)
10 empty empty
13. Electrophorese for 30 minutes at 200 V in 1x
TGS gel running buffer.
14. Proceed directly to Lesson 3, continue to step 15
of Lesson 2 to stain gels or store gels overnight
at 4°C.
15. If the gels are to be stained, save 50 ml of 1x TGS
gel running buffer.
16. Remove gel from cassette and transfer gel to a
container with 25 ml Bio-Safe Coomassie
stain/per gel and stain gel for 1 hour, with gentle
shaking for best results.
17. Discard stain and destain gels in a large
volume of water for at least 30 minutes to
overnight, changing the water at least once.
Blue-stained bands will be visible on a clear gel
after destaining.