Bio-Rad Fish DNA Barcoding Kit - Manual (Page 8)

6 Fish DNA Barcoding Quick Guide
Preparing PCR Samples for
Electrophoresis and Sequencing
1. Label four 2 ml microcentrifuge tubes with both
your initials and E. E stands for electrophoresis.
Now label one of these tubes Fish 1, one tube
Fish 2, one tube (+), and one tube (–).
2. Remove 5 µl from each PCR reaction and
deposit into the 2 ml microcentrifuge tube
corresponding to that sample.
3. Label three 2 ml microcentrifuge tubes with
both your initials and SEQ. SEQ stands for
sequencing. Now label one of these tubes
Fish 1, one tube Fish 2, and one tube (+). You
will not be sequencing your negative control
sample.
4. Remove 30 µl from each PCR reaction and
deposit into the 2 ml microcentrifuge tube
corresponding to that sample.
5. Store all samples at 4°C until you are ready to
proceed with electrophoresis and sequencing.
1, PCR
Product 1, SEQ
Initials
30 µl
1, PCR
Product
1, E
Initials
5 µl
1, E
Initials
2, E
Initials
(+), E
Initials
(–), E
Initials
1, SEQ
Initials
2, SEQ
Initials
(+), SEQ
Initials