Bio-Rad Fish DNA Barcoding Kit - Manual (Page 3)

Fish DNA Barcoding Quick Guide 1
Quick Guide
Lesson 1: DNA Extraction
Preparing Fish Samples
1. Label one capped 2 ml microcentrifuge tube for
each of your fish samples (that is, “1” for fish
sample 1, “2” for fish sample 2, etc.). Also label
with your initials.
Fish 1__________________
Fish 2__________________
2. Cut a piece of fish muscle up to 100 mg in
mass, approximately the size of a pencil eraser-
head, from your first fish sample. Place the piece
in a new weigh boat and slice it with a razor
blade or cutting implement until finely minced.
Transfer the sample into the appropriately
labeled microcentrifuge tube.
3. Properly discard the razor blade or cutting
implement. If wearing gloves, change gloves
before handling the next piece of fish. If not,
wash hands thoroughly.
4. Using a new razor blade or cutting implement,
cut a piece of fish muscle up to 100 mg in mass,
approximately the size of a pencil eraser-head,
from your second fish sample. Place the piece
in a new weigh boat and slice it with a razor
blade until finely minced. Transfer the sample
into the appropriately labeled microcentrifuge
tube. Properly discard the razor blade or cutting
implement.
Initials Initials
1
2
1
2