Versafluor filter selection guide, Helpful hints – Bio-Rad VersaFluor™ Fluorometer User Manual
Page 2
4100157 Rev A
VersaFluor Filter Selection Guide
Excitation
Catalog
Emission
Catalog
Application
Filters
Number
Filters
Number
DNA Quant (Hoechst 33258)
EX 360/40 (340–380 nm) 170-2420
EM 460/10 (455–465 nm) 170-2421
ß-Galactosidase (MUG)
EX 360/40 (340–380 nm) 170-2420
EM 460/10 (455–465 nm) 170-2421
ß-Glucuronidase (MUGluc)
EX 360/40 (340–380 nm) 170-2420
EM 460/10 (455–465 nm) 170-2421
Apopain (Z-DEVD-AFC)
EX 360/40 (340–380 nm) 170-2420
EM 520/10 (515–525 nm) 170-2424
pH indicator (BCECF)
EX 490/10 (485–495 nm) 170-2422
EM 520/10 (515–525 nm) 170-2424
Calcium indicator (Fluo-3)
EX 490/10 (485–495 nm) 170-2422
EM 520/10 (515–525 nm) 170-2424
DNA Quant (PicoGreen™)
EX 480/20 (470–490 nm) 170-2427
EM 520/10 (515–525 nm) 170-2424
Oligo Quant (OliGreen™)
EX 480/20 (470–490 nm) 170-2427
EM 520/10 (515–525 nm) 170-2424
Protein Quant (NanoOrange™) EX 480/20 (470–490 nm) 170-2427
EM 590/10 (585–595 nm) 170-2425
Fluorescein
EX 490/10 (485–495 nm) 170-2422
EM 520/10 (515–525 nm) 170-2424
Ethidium Bromide
EX 510/10 (505–515 nm) 170-2423
EM 620/10 (615–625 nm) 170-2426
RNA Quant (RiboGreen™)
EX 490/10 (485–495 nm) 170-2422
EM 520/10 (515–525 nm) 170-2424
Helpful Hints
Use filters which are tailored to the sample being measured. Using incorrect
filters will greatly degrade the accuracy of the fluorescence measurement.
• Always use calibrated pipets to insure accurate pipetting.
• Mix standards and sample completely by using a disposable transfer
pipet.
• Remove any air bubbles in the cuvettes.
• Hold fluorometer cuvettes by the upper edges since the cuvettes have
optically clear sides.
• Clean the cuvette sides with a lint-free tissue.
• Read all standards and samples at ambient temperature.
• While taking a reading, keep the samples in the fluorometer only. This
helps reduce sample photobleaching.
• Fluorescence measurements can be made continuously. It is not nec-
essary to zero the instrument before each measurement unless the
baseline conditions change. Check the zero of the instrument with the
baseline fluid (blank) when switching between gain settings.
PicoGreen, OliGreen, NanoOrange, and RiboGreen are trademarks of Molecular Probes Inc.
Bio-Rad Laboratories, 2000 Alfred Nobel Drive, Hercules, CA 94547
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