Bio-Rad VersaFluor™ Fluorometer - Manual

Note: The instrument should be allowed to warm up for at least 20 minutes before
taking any readings.
1. Selection and insertion of two optical filters.
Insert the emission and excitation filters into the VersaFluor
Fluorometer.
2. Set the gain.
Press the SETUP button on the main menu and set the gain to LOW,
MED, or HIGH.
3. Zero the instrument.
Set the range to 00000 by pressing the RANGE button. Adjust the
range to 00000. Press EXIT. Place the baseline sample cuvette in the
cuvette chamber and press the SET ZERO button. When the blinking
stops, the fluorescence display should read 0 ± 5. If not, re-zero the
instrument by pressing the SET ZERO button.
4. Set the range.
Set the range by placing a cuvette containing the highest concentra-
tion standard in the cuvette holder and closing the sample compart-
ment lid. Wait approximately 10 seconds for the detector to adjust to
the light conditions. Press the RANGE button. Adjust the range to the
desired setting. The maximum setting is 19,999 RFU. Press EXIT.
5. Measure the sample.
Place a sample cuvette in the cuvette holder and close sample com-
partment lid. Wait approximately 10 seconds for the detector to adjust
to the light conditions. Record or print the RFU value in the fluorescence
display. Read all remaining samples.
VersaFluor
™
Quick
Reference Guide
RANGE SET
PRINT 00000 ZERO SETUP
AVRG GAIN
1 SEC LOW MORE EXIT
SET FULL RANGE: 00000
UP DOWN —> EXIT
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