Bio-Rad RC DC™ Protein Assay - Manual (Page 2)

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Section 1 Introduction
The RC DC Protein Assay is a colorimetric assay for protein quantitation with all
the functionality of the original DC Protein Assay. This assay is based on the
Lowry1 assay but has been modified to be reducing agent compatible (RC) as well
as detergent compatible (DC).
Section 2 Product Description
RC Reagents Package, includes
• RC Reagent I (250 ml)
• RC Reagent II (250 ml)
(Sufficient for 500 standard assays or 2,000 microfuge tube assays)
RC Reagent I contains UPPA-I
RC Reagent II contains UPPA-II
UPPA is a trademark of Geno Technology, Inc.
Section 3 Reagent Compatibility
The listed reagents were tested and found to be compatible with the RC DC
Protein Assay. The presence of one or more of these substances may change the
response of the protein to the assay reagents. Thus the protein standard should
always be prepared in the same buffer as the protein sample.
Reagents One Wash Two Washes (Optional)
Dithiothreitol (DTT) 100 mM 350 mM
Tributylphosphine (TBP) 2 mM -
β-mercaptoethanol 5% 10%
Sequential Extraction Buffer 2♦ Not Compatible Full Strength
Sequential Extraction Buffer 3♦♦ Not Compatible Full Strength
Laemmli Buffer
(with 5% β-mercaptoethanol) Full Strength -
CHAPS 2% -
Tween 20* 2% -
Triton X-100** 2% -
EDTA 100 mM -
Imidazole 500 mM -
Tris, pH 8.4 500 mM -
NaOH 2.5 M -
*Tween is a registered trademark of ICI Americas, Inc.
**Triton is a registered trademark of Rohm and Haas.
♦40 mM Tris, 8 M urea, 4% (w/v) CHAPS, 0.2% (w/v) Bio-Lyte 3/10 ampholyte, 2 mM TBP
(Catalog #163-2103)
♦♦40 mM Tris, 5 M urea, 2 M thiourea, 2% (w/v) CHAPS, 2% (w/v) SB 3-10, 0.2% (w/v)
Bio-Lyte 3/10 ampholyte, 2 mM TBP (Catalog #163-2104)
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