Bio-Rad Rotofor® and Mini Rotofor Cells - Manual (Page 4)

Section 2
Starter Kit Check List
This kit contains:
1. Bio-Lyte ® Ampholytes
10 ml, pH range 3/10
2. Protein Sample (in 1 ml vial)–Catalog Number 170-2919
Phycocyanin, 2 mg Color: Blue (p I range of visible subunits is 4.5-5.5)
Hemoglobin, 2 mg Color: Red (pI range of visible subunits 6.0-7.5)
Cytochrome c, 2 mg Color: Orange (pI range of visible subunits is 8.0-9.0)
3. Ion Exchange Membranes
Anion Exchange Membrane, 1, in cathode electrolyte solution 0.1 M NaOH,
33 ml
Cation Exchange Membrane, 1, in anode electrolyte solution 0.1 M H
3
PO
4
,
33 ml
4. Vent Buttons, 2
5. Sample Loading Syringe and Needle, 50 ml
Required Equipment and Reagents:
Rotofor cell
3,000 volt power supply
Water recirculation chiller
Vacuum source
Approximately 60 ml dH
2
O
Beaker, 100 ml
Pipettes 1 ml, for preparing the sample
2
LIT603B 7/10/98 11:56 AM Page 2