Bio-Rad Rotofor® and Mini Rotofor Cells - Manual (Page 13)

2. After about 1 hour, the proteins began to concentrate near their respective
isoelectric points during initial formation of the pH gradient.
3. After the voltage stabilized, the proteins focused at their isoelectric points.
The total run took approximately 2 hours.
4. Following harvesting, test tube fractions may be measured for pH to confirm
the linearity of the pH gradient.
Step 10: Disassembly and Cleaning
1. Rinse the needle array and its associated tubing with water as soon as possi-
ble after use.
2. Take the focusing chamber from the stand. Loosen the nylon screws and
remove the cathode chamber.
3. Leave the cathode and anode chambers intact. The ion exchange membranes
must be stored wet. Remove the electrolyte and fill the electrode chambers
with distilled water. Before starting a new run, the electrolytes must be
replaced with fresh solutions.
4. Loosen the nylon screws on the anode chamber and remove the focusing
chamber and membrane core. Rinse all chamber components with water
and air dry.
Section 4
Product Information
Catalog
Number
Product Description
170-2919 Protein Sample for Rotofor System Starter Kit, 1 ml
Bio-Lyte Ampholytes
163-1112 Bio-Lyte 3/10 Ampholyte, 40%, 10 ml
163-1132 Bio-Lyte 3/5 Ampholyte, 20%, 10 ml
163-1142 Bio-Lyte 4/6 Ampholyte, 40%, 10 ml
163-1152 Bio-Lyte 5/7 Ampholyte, 40%, 10 ml
163-1192 Bio-Lyte 5/8 Ampholyte, 40%, 10 ml
163-1172 Bio-Lyte 6/8 Ampholyte, 40%, 10 ml
163-1182 Bio-Lyte 7/9 Ampholyte, 40%, 10 ml
163-1113 Bio-Lyte 8/10 Ampholyte, 20%, 25 ml
163-1143 Bio-Lyte 4/6 Ampholyte, 40%, 25 ml
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