Bio-Rad ReadyPrep™ Protein Extraction Kit (Membrane II) - Manual (Page 9)

Lysis Buffer. Empty the entire contents of one pouch of
Lysis Buffer into a 50 ml disposable tube. Add 50 ml of
ReadyPrep proteomic grade water or similar quality water
to the tube and mix until the solids are completely
dissolved. Rinse the pouch with a small portion of this
buffer, and return the solution to the 50 ml tube. If desired,
protease inhibitors can be added to a portion of this buffer
just before use.
Membrane Protein Concentrating Reagent. Empty
the entire contents of one pouch of Membrane Protein
Concentrating Reagent into a beaker or bottle containing
125 ml of ReadyPrep proteomic grade water or similar
quality water. To speed up dissolving the solid, add the
contents of the pouch while the water is stirring. Mix until
all the solids are completely dissolved. Rinse the pouch
with a small amount of the solution (10–15 ml) and then
return it to the container. Protease inhibitors can be added
to a portion of this buffer just before use if desired.
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