Bio-Rad ReadyPrep™ Protein Extraction Kit (Membrane II) User Manual
Page 8
Section 4
Reagent Preparation
ReadyPrep 2-D Rehydration/Sample Buffer 1
:: Add
5.6 ml of ReadyPrep proteomic grade water or similar
quality water to one bottle. Swirl the vial gently until the
contents are completely dissolved. The solution can be
warmed slightly in the palm of the hand or in a water bath
to speed the dissolution process. DO NOT heat the
solution above 25 to 30°C to avoid the formation of
cyanates. Cyanates can react with and modify the proteins
in the sample.
ReadyPrep TBP Reducing Agent. Tributylphosphine
(TBP) has an unpleasant odor and is very volatile. Work
with TBP in a fume hood. Wear a laboratory coat and
gloves when handling the ampoule of TBP reducing agent.
Wipe up spills with wet towels. Open the ampoule by
snapping the top off at the scored neck. Transfer the entire
contents of the ampoule to the empty screw-cap storage
vial provided. Screw the cap of the vial down tightly and
store the vial at –20°C to prevent evaporation of the TBP.
While using, keep the vial of TBP reducing agent on ice.
6
4110157A.qxd 6/16/2003 2:21 PM Page 9