Bio-Rad ReadyPrep™ Protein Extraction Kit (Membrane II) - Manual (Page 6)

Items Required But Not Provided
• 2.0 ml microcentrifuge tubes (for example, VWR catalog
#20170-237).
• Disposable round-bottom tubes for plant sample
extraction (for example, VWR catalog # 60818-725).
• ReadyPrep 2-D cleanup kit (Bio-Rad catalog #163-2130)
for plant sample extraction. When working with plant leaf
tissue, removal of interfering compounds, such as
phenolics, is essential for high quality 2-D gels with
minimal horizontal streaking.
• 50 ml conical centrifuge tube(s), disposable, (for
example, VWR catalog #20171-030).
• 2 clean 125–150 ml beakers or bottles plus stirring bars.
• Ultracentrifuge, rotor and tubes capable of spinning at
100,000 x g. Typically, tubes should have ~30 ml
capacity.
• Microcentrifuge capable of spinning at 12–16,000 x g
at 4°C.
• Sonicator
• Benzonase (endonuclease) (for example, Sigma
catalog #E1014 or #E8263)
• 1 M MgCl
2
solution
• Protease inhibitor(s) (optional)
• Carrier ampholytes (for example, Bio-Lyte
 3/10
ampholyte, Bio-Rad catalog #163-2094)
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