Bio-Rad ReadyPrep™ Protein Extraction Kit (Membrane II) User Manual
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Section 2
Kit Specifications
Each ReadyPrep protein extraction kit (membrane II)
provides sufficient reagents to perform 10 extractions each
starting with 100–200 mg of cells or tissue. The procedure
can easily be scaled up to accommodate larger amounts of
cells or tissue. The entire procedure can be completed in
less than 3 hr.
Items Supplied With Kit
• 2-D Rehydration/Sample Buffer 1. 2 vials. Lyophilized.
After reconstituting, each vial contains 10 ml of 7 M urea,
2 M thiourea, 1 % (w/v) ASB-14 detergent, 40 mM Tris
base, and 0.001% Bromophenol Blue.
• Lysis Buffer. 5 pouches. Each pouch reconstitutes to
make 50 ml of Lysis Buffer.
• Membrane Protein Concentrating Reagent. 5 pouch-
es. Each pouch reconstitutes to make 125 ml of mem-
brane protein concentrating reagent.
• TBP Reducing Agent. 1 ampoule containing 0.6 ml of
200 mM tributylphosphine (TBP) in 1-methyl-
2-pyrrolidinone sealed under nitrogen gas.
• Empty Vial. 1 storage vial for TBP reducing agent.
• Instruction Manual, 1.
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