Bio-Rad ReadyPrep Protein Extraction Kit (Total Protein) User Manual
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• ReadyPrep 2-D cleanup kit (Bio-Rad catalog #163-2130)
for plant sample extraction. When working with plant leaf
tissue, removal of interfering compounds, such as
phenolics, is essential for high quality 2-D gels that are
free of horizontal streaks.
• Microcentrifuge capable of spinning at 12–16,000 x g
while maintaining a temperature between 18–20°C.
• Sonicator
• Protease inhibitor(s) (optional)
• Carrier ampholytes (for example, Bio-Lyte
3/10
ampholyte, Bio-Rad catalog #163-2094)
• ReadyPrep proteomic grade water (Bio-Rad catalog
#163-2091).
• RC DC
™
Protein Assay (Bio-Rad catalog #500-0121 or
#500-0122)
Section 3
Storage Conditions
Store the unopened kit at room temperature. After opening,
unused reconstituted ReadyPrep 2-D Rehydration/Sample
Buffer 1 should be aliquoted in 1 to 2 ml volumes and
stored frozen at –80°C. After opening, transfer the
ReadyPrep TBP reducing agent to the empty glass vial
provided and store the vial at –20°C to prevent evaporation
of the TBP.
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