Bio-Rad ReadyPrep Protein Extraction Kit (Total Protein) - Manual (Page 3)

Section 1
Introduction
The ReadyPrep protein extraction kit (Total Protein) is
designed as a simple, rapid and reproducible method to
prepare total cellular protein extracts suitable for 2-D gel
analysis. The strongly chaotropic extraction solution used in
the kit contains the zwitterionic detergent ASB-14, making
this solution one of the most powerful solubilizing reagents
available for 2-D electrophoresis. The extraction process
solubilizes many types of cell proteins, including membrane
proteins, and the extraction protocol can be applied to a
wide variety of biological samples, from animal cells and
tissues to yeast, bacteria and plant tissues. At the end of
the extraction process, the protein sample can be applied
onto Immobilized pH gradient strips (IPGs) and subjected
to isoelectric focusing and 2-D gel separation.
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