Bio-Rad ReadyPrep Protein Extraction Kit (Total Protein) User Manual
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7.
Determine the protein concentration of the sample.
The Bio-Rad RC DC Protein Assay (catalog #500-
0121 or #500-0122) is recommended for this
measurement. This assay allows for accurate protein
quantitation in the presence of detergents, reducing
agents, and other substances that typically interfere
with other protein assays.
8.
An appropriate dilution of the protein extract in 2-D
rehydration/sample buffer 1 containing TBP reducing
agent and ampholytes may be needed before IEF/2-D
gel analysis. Refer to Section 6 for guidelines on
selecting the appropriate volume to use. Additional
ReadyPrep 2-D rehydration/sample buffer 1 is
available and sold separately for this purpose (see
Section 7).
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