Bio-Rad ReadyPrep Protein Extraction Kit (Total Protein) User Manual
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Section 2
Kit Specifications
Each ReadyPrep protein extraction kit (Total Protein)
provides sufficient reagents to perform up to 20 extractions
each starting with 50–100 mg of cells or tissue. The
procedure can easily be scaled up to accommodate larger
amounts of cells or tissue. The entire procedure can be
completed in about 45 min.
Items Supplied With Kit
• 2-D Rehydration/Sample Buffer 1. Two vials.
Lyophilized. After reconstituting, each vial contains 10 ml
of 7 M urea, 2 M thiourea, 1 % (w/v) ASB-14 detergent,
40 mM Tris base, and 0.001% Bromophenol Blue.
• TBP Reducing Agent. 1 ampoule containing 0.6 ml of
200 mM tributylphosphine (TBP) in 1-methyl-
2-pyrrolidinone sealed under nitrogen gas.
• Empty Vial. 1 storage vial for TBP reducing agent.
• Instruction Manual, 1.
Items Required But Not Provided
• 2.0 ml microcentrifuge tubes (for example, VWR catalog
#20170-237).
• Disposable round-bottom tubes for plant sample
extraction (for example, VWR catalog #60818-725).
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