beautypg.com

2 kit contents – Bio-Rad Quantum Prep Plasmid Kits User Manual

Page 6

background image

Plasmid yield depends on a number of factors such as the

vector copy number, culture volume and aeration, bacterial strain,
and growth media. The Quantum Prep kit was optimized using
high copy number plasmids grown in rich media such as Terrific
Broth.

2

The Quantum Prep kit permits yields as high as 25 µg from

a 1.5 ml culture of DH5αF'[pTZ18U] grown in Terrific Broth. Yields
as high as 40 µg have been obtained with other plasmids using
the Quantum Prep kit.

1.2 Kit Contents

The Quantum Prep Miniprep kit contains reagents sufficient for

100 plasmid minipreps.
25 ml

Cell Resuspension Solution

25 ml

Cell Lysis Solution

25 ml

Neutralization Solution

20 ml

Quantum Prep Matrix

63 ml

Wash Buffer

100

Spin Filters

100

Wash Tubes

100

Collection Tubes

2