Bio-Rad Quantum Prep Plasmid Kits - Manual (Page 12)

8
30
25
20
15
10
5
0
450
400
350
300
250
200
150
100
50
Elution volume, µl
DNA yield
DNA concentration
0
25
DNA concentration, ng/µl
DNA yield, µg
50 75
100 125 150 175 200
Fig. 1. Effect of elution volume on DNA concentration and yield
from a 1.5 ml culture of DH5
αF'[pTZ18U] grown in Terrific Broth
and purified using the Quantum Prep Plasmid Miniprep kit.