Bio-Rad Chromatography Standards - Manual (Page 2)

For Calibration of
Hydrophobic Columns
The Protein Standard for Hydrophobic Chroma-
tography is a lyophilized mixture of proteins which
exhibit different hydrophobic behavior. It may be used as
a calibration standard for HIC columns for analysis under
nondenaturing conditions, and is appropriate for
calibrating some reverse phase columns as well. The
mixture contains cytochrome c, myoglobin and
lysozyme. The standard is supplied as a set of 6 vials of
lyophilized protein mixture. The total protein content is
approximately 3 mg per vial.
Instructions
Rehydrate the standard by adding 0.5 ml of starting
buffer to the vial. Swirl gently to mix and allow the vial
to stand for 2–3 minutes. Swirl the vial again; centrifuge
or filter to remove fine particulates before injection.
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