Bio-Rad Bio-Beads SM-2 Adsorbents - Manual (Page 5)

slurry is necessary to fill the column. Use wet
density information from Table 1 to calculate the
quantity of adsorbent needed for size of the column.
2. Allow the slurry mixture to flow down the side of
the column so that a minimal amount of air bubbles
will be introduced into the chromatographic media
bed.
3. Allow the adsorbent to settle, and elute excess
solvent from the bottom of the column. Make certain
that the solvent level does not go below the top of
the adsorbent bed, because this will allow air bubbles
to form on the top of the column.
4. Equilibrate the Bio-Beads SM adsorbent with 3 bed
volumes of your desired buffer (ex. 10 mM
potassium phosphate, pH 7.2).
5. Apply sample to the column.
6. Wash with 1–2 bed volumes of buffer at 0.3 ml/min.
7. The column can be regenerated by washing in bed
volumes of 100% methanol, followed by rinsing in
DI water.
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A complete list of more than 100 applications for
Bio-Beads adsorbents can be obtained by requesting
technical bulletin 1461.
Section 3
Instructions for Use
3.1 Preparation of Bio-Beads SM
Adsorbent (Bulk)
Due to the macroporous nature of the adsorbent, air
will sometimes become trapped within the pores. As a
result, it will float in solution. To correct this, slurry the
adsorbent in the solution you will use later. Degas the
slurry by placing it in a vacuum flask and pulling a
vacuum while stirring or sonicating at the same time.
Decant the excess elution solvent. The adsorbent is then
ready for use, either in the batch method or column
method.
3.2 Column Method
1. Slurry the Bio-Beads SM adsorbent in the solvent
that will be used in the process. Usually a 66-75%
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