Bio-Rad Bio-Gel P Polyacrylamide Gel - Manual (Page 4)

Section 2
Technical Description
Table 1. Bio-Gel P Gel Product Description
Matrix Bio-Gel polyacrylamide gel
Particle size
Medium 90-180 µm
Fine 45-90 µm
Extra fine < 45 µm
Shipping medium Shipped dry
Resistance
pH 2-10
Pressure 15 psi
Organic solvents < 20%
Working temperature range 4-80 °C
Temperature limits Autoclavable, at pH 5.5-6.5, at
120 °C for 30 min
Storage Dry, at room temperature; in dis-
tilled water or aqueous buffers
at 4 °C with 0.02% sodium azide
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exclusion properties of the gel, and will in some cases enhance
separation of complex mixtures of poorly water soluble small
molecules such as nucleotides, peptides, and tannins. Formamide
may be used at full strength, because Bio-Gel P gel is completely
swelled by this solvent.
Bio-Gel P gel is autoclavable at pH 5.5-6.0 in buffers such as
50 mM HEPES, MES, or citrate at 120 °C for 15-30 minutes. At
room temperature, the recommended operating pH range is 2-10.
Bio-Gel P gel is susceptible to hydrolysis of amide groups at higher
or lower pH. Flow rate and resolution increase with temperature over
the range of 4-80 °C.
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LIT174B 10/13/98 9:26 AM Page 2