Bio-Rad Bio-Gel® HTP Hydroxyapatite User Manual
Page 3
Section 1
Properties of Bio-Gel HT, HTP,
and DNA Grade HTP
Hydroxyapatite
Hydroxyapatite, a crystalline form of calcium phos-
phate, is widely used in preparative biochemistry, having
proven itself a unique tool for the fractionation and
purification of monoclonal antibodies
1-7
and other pro-
teins,
8,9
enzymes,
10-12
and nucleic acids.
13-18
Hydroxyap-
atite is useful for preparative work in column or batch
modes, and for quantitative analysis of proteins or nucle-
ic acids. Its advantages include:
Unique selectivity - Since molecular separation on
hydroxyapatite is not primarily dependent on molecular
weight, molecular size, charge density, or isoelectric
point, hydroxyapatite chromatography is a valuable com-
plement to other separations techniques.*
1
*
For a discussion of the mechanism of action of hydroxyapatite,
refer to the publications of M. J. Gorbunoff.
19-21
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