Bio-Rad AG® MP-50 Cation Exchange Resins - Manual (Page 6)

Section 5
Reference
1. Rodriquez, R. L. and Tait, R. C., Recombinant DNA
Techniques: An Introduction, Addison-Wesley Publishing
Co., Reading, Massachusetts, 153-158 (1983).
Section 6
Product Information
Wet Density
Catalog Mesh Ionic Pkg. Capacity Diameter (Nominal)
Number Size Form Size (meq/ml) (microns) gm/ml
Molecular Biology Grade AG 50W-X8 Resin
143-1441 100-200 Sodium 100 g 1.7 106-250 0.80
143-1451 200-400 Sodium 100 g 1.7 63-150 0.80
Columns
731-1550 Poly-Prep ® Columns , empty, 50
731-7005 Poly-Prep Column Rack , 1
7
4. Dilute DNA with an equal volume of A-50 buffer
and apply the DNA sample to the column. Dilution
of the sample is necessary to slow the flow rate and
allow complete removal of the propidium or
ethidium. Use a stopcock to slow the flow rate if
necessary.
5. Add 1 bed column (1.5 ml) of A-50 buffer to wash
out any remaining DNA.
6. Check DNA sample with UV to insure that the dye
has been removed. If not, remove the top layer of the
resin containing the dye, discard, and run the DNA
sample over the column again.
Warning: Ethidium and propidium are carcinogenic.
The used columns should be disposed of as hazardous
waste.
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