Bio-Rad Macro-Prep 25 S Media - Manual (Page 5)

Table 1. Properties of Macro-Prep Ion Exchange Supports.
Macro-Prep 25 Q Macro-Prep 25 S
Support Support
Type of ion exchanger Strong anion Strong cation
Functional group –N+(CH
3
)
3
–SO
3
–
Ionic capacity 220 ± 40 µeq/m 110 ± 30 µeq/ml
Dynamic binding capacity >30 mg/ml BSA >40 mg/ml BSA
Shipping counterion Cl
–
Na+
Nominal particle size 25 µm 25 µm
Nominal pore size 725 Е 725 Е
Recommended linear 50–300 cm/hr 50–300 cm/hr
flow rate range
Maximum linear 3,000 cm/hr 3,000 cm/hr
flow rate
Volume changes
pH 4–10 <1% <1%
0.1–1.0 M NaCl <5% <5%
Autoclavable (121°C, 30 min) Yes* Yes
pH stability 1–10 1–12
Storage condition 20% ethanol 20% ethanol
*Do not autoclave in the OH – form
Chemical Stability
Macro-Prep 25 ion exchange resins are stable in most aqueous buffer solutions
commonly used in purification of biomolecules. These ion exchangers also
withstand treatment in solutions of acid, chaotropic agents, detergents, and
pH <10, while retaining full functional performance. We do not recommend routine
cleaning or operation of Macro-Prep 25 Q above pH 10. Macro-Prep 25 ion
exchange supports should not be sanitized or stored in NaOH.
Thermal Stability
The Macro-Prep 25 supports can be autoclaved at 121°C for up to 30 min in
deionized water as a slurry or a moist cake. Do not autoclave Macro-Prep 25 Q in
OH
–
form.
3
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