Bio-Rad Affi-Gel Blue Gel - Manual (Page 12)

Table 2. Affi-Gel Blue Gel Enzyme Applications
(continued)
Enzyme Source Eluant Reference
Glutamine synthetase Salmonella typhimurium 5 mM ATP 8
Glyoxalase II Rat erythrocytes 0-0.2 M KCl 19
GMP reductase Human erythrocytes 1 mM NADPH, 2 mM GMP 21
GTP:RNA guanylyltransferase Wheat germ 0.05-0.75 M NaCl 5
Isocitrate dehydrogenase E. coli 2 mM NADP 16
Isocitrate dehydrogenase E. coli 2 mM NADP+ 13
MB creatine kinase Human heart 0.25 M NaCl 7
Membrane-bound Rat brain Triton X-100, NaCl, glycerol 38
phosphatidylinositol kinase
Methylenetetrahydrofolate
Porcine liver 0-10 mM NADPH 17
reductase
Metmyoglobin reductase
Bovine heart 1.0 M NaCl, 1 mM NADP+ 11
Phosphodiesterase Bovine brain 0.2 M NaCl 25
Replication protein A (RP-A) Animal cells - 40
RNA ligase E. coli 0.2 M NaCl, 2 mM ATP 20
Serine transhydroxymethylase Porcine liver 0.5 M KCl 18
Thymidylate Saccharomyces cervisiae 0.25-1 M KCl 22
Tyrosine phenollyase Erwinia herbicola 1.5 M NaCl, 0.5 mM mercaptoethanol 9
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LIT590B 9/3/98 1:30 PM Page 10