Bio-Rad Profinity Epoxide Resin - Manual (Page 4)

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** Maximum pressure test: Profinity epoxide resin packed in a 1.1 x 30 cm Amicon column to a bed height
of 20 cm with 20 mM sodium phosphate buffer up to 43 psi (3 bar). Flow rates were increased stepwise
to 200 cm/h and held for 2 min at each step. The pressure-low curve for Profinity epoxide becomes
nonlinear at pressures above 80 psi.
**** Refers to base matrix of coupled resin. The stability of coupled ligands may be a limitation of an affinity
resin’s stability.
Functional group Epoxy group
Base matrix UNOsphere base matrix
Form Dry powder
Particle size 45–90 µm
Mean particle size 60 µm
Functional group density 50–132 µmol/g UNOsphere epoxide resin
Swelling factor (ml drained resin/g resin) 5.5–8.0
Recommended linear flow rate <600 cm/hr at 25°C
Maximum operating pressure (net)* ≥ 80 psi
pH stability (base matrix of coupled resin)** 1–14
Chemical compatibility (base matrix
of coupled resin)** Compatible with common buffers
and aqueous solutions
Storage 4°C ambient temperature
Shelf life > 1 year at ambient temperature
Operational temperature 4–40°C
Autoclavability (base matrix
of coupled resin)**
0.1 M sodium acetate at 120°C for 30 min
Table 1: Characteristics of Profinity epoxide resin
Profinity epoxide
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