Bio-Rad Bio-Scale™ Mini Macro-Prep® High Q and Macro-Prep High S Cartridges - Manual (Page 23)

appropriate cleaning protocol. As a general guide, we
recommend the following:
1. Use high salt buffer for regeneration, as above.
2. For aggregated or precipitated proteins, or
when dirty feed stock (e.g., crude lysate) has
been used, wash with 3–5 column volumes of
6 M guanidine-HCl or 8 M urea at 100 cm/hr.
3. For lipids or hydrophobically bound contaminants,
wash with 0.1% Triton X-100 or 20–70%
ethanol or isopropyl alcohol, or 1–30% acetic
acid. Use 3–5 column volumes at 100 cm/hr.
4. Remove additional contaminants with 0.4 M
NaCl in 1% acetic acid/1% phosphoric acid.
Use 3–5 column volumes at 100 cm/hr.
5. If the cartridge is to be used again immediately,
wash with 2 column volumes of deionized
water and 4–5 column volumes of starting
buffer at 100 cm/hr. Check the conductivity
and pH of the effluent to verify that the column
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