Bio-Rad Bio-Scale™ Mini Macro-Prep® High Q and Macro-Prep High S Cartridges - Manual (Page 20)

gradient from 0.0 to 0.4 M NaCl spanning 1 to 20
column volumes at 120 cm/hr, or 0.5 ml/min for the
1 ml cartridge and 2.5 ml/min for the 5 ml cartridge.
The separation can be optimized by changing the
gradient profile. At the end of each run, the cartridge
can be regenerated with 1.0 M NaCl followed by
starting buffer. Return to the desired flow rate and
proceed with the next separation.
4.3 Scaling Up the Separation
For quick scale-up, two or three cartridges of the
same type can be connected in series.
Backpressure will increase with cartridges in series,
so care should be taken to maintain pressures
≤45 psi.
Bio-Scale Mini cartridges are available in 1 ml and
5 ml cartridge formats. The Macro-Prep High Q,
High S, and DEAE ion exchange media are also
available in larger amounts, from 25 ml bottles to
bulk quantities, for scaling up methods developed
using the cartridges. Macro-Prep High Q, High S,
and DEAE media are fully supported with Regulatory
Support Files. In addition, Bio-Rad carries an
extensive line of empty chromatography columns
from laboratory scale to process scale.
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