Bio-Rad DEAE Affi-Gel Blue Gel - Manual (Page 3)

Section 1
Econo-Pac Serum IgG Purification Kit
Catalog Number 732-3037
1.1 Introduction
The Econo-Pac serum IgG purification kit provides everything necessary for
fast and convenient purification of IgG from serum. Serum sample preparation
is greatly simplified by using the Econo-Pac 10DG desalting columns, which
eliminate the need for lengthy membrane dialysis procedures before chro-
matography. Prepacked DEAE Affi-Gel
® blue columns allow the purification of
IgG from other serum proteins and plasminogen. The IgG fraction obtained
will have some residual contamination from transferrin. Buffers are provided pre-
mixed and preweighed for easy preparation.
1.2 Kit Components
Econo-Pac serum IgG Five Econo-Pac columns packed with 10 ml
purification columns of DEAE Affi-Gel blue gel
Econo-Pac 10DG Five Econo-Pac 10DG desalting columns.
desalting columns
Human application buffer One bottle (13 g) of buffer solids,
reconstitution volume = 3,000 ml
Rabbit application buffer One bottle (13 g) of buffer solids,
reconstitution volume = 3,000 ml.
Regeneration buffer One bottle (121 g) of buffer solids, reconsti-
tution volume = 1,000 ml. Caution:
Contains sodium thiocyanate. Avoid skin
contact. Avoid breathing dust or fumes.
1.3 Additional Items Required But Not Provided
Column rack The Econo-Pac 10 (12 place) acrylic rack is ideal for holding
the Econo-Pac columns. Other benchtop or lattice mount
racks may also be used.
Test tubes General purpose tubes for fraction collection are recommended.
pH meter A pH meter is required to check the pH of the human and
rabbit buffers after reconstitution.
Mixer Standard laboratory magnetic stirrer and bar for buffer mixing.
Balance Standard laboratory scale for weighing out buffer solids.
Filters 0.45 micron filters for buffer preparation.
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LIT154B 9/2/98 9:02 AM Page 1