Bio-Rad Bio-Scale™ CHT™ Type I Columns - Manual (Page 2)

Table of Contents
Page
Section 1 Characteristics of the Bio-Scale CHT2, CHT5, CHT10, CHT20
Ceramic Hydroxyapatite, Type I Columns ...............................................1
1.1 Introduction ................................................................................................................1
1.2
The CHT-I Separation Medium .................................................................................1
1.3 Connection to the BioLogic, FPLC ® and HPLC Systems ........................................1
Section 2 Use of the Bio-Scale CHT-I Columns.........................................................2
2.1 Preparation for Initial Use ..........................................................................................2
2.2 Sample Preparation ....................................................................................................3
2.3 Elution Conditions......................................................................................................3
2.4 Use of Detergents .......................................................................................................4
2.5 Effect of Calcium Chloride ........................................................................................4
2.6 Chromatography Pre-Runs.........................................................................................4
Section 3 Care of the Bio-Scale CHT-I Columns ......................................................5
3.1 Column Hygiene ........................................................................................................5
3.2 Bed Height Adjustment..............................................................................................5
3.3 Frit Removal...............................................................................................................5
3.4 Top-Off Resin.............................................................................................................5
3.5 Storage Conditions .....................................................................................................5
3.6 References ..................................................................................................................8
Section 4 Product Information....................................................................................8