Bio-Rad UNO® Monolith Cation Exchange Columns - Manual (Page 2)

UNO Q & S Polishing Ion Exchange Column
Introduction
UNO prepacked ion-exchange columns are designed to meet the needs of the
bio-chromatographer for rapid and reproducible high-resolution separations of
biomolecules including proteins, peptides and polynucleotides. The UNO Polishing
column is designed specifically as a late-stage purification tool to obtain the highest
resolution and recovery from a small sample load (up to 2 mg). The small column
volume means that peak elution volumes are usually 0.2 ml or less. This presents an
unique tool to purify and concentrate dilute samples in one step. However, the pol-
ishing column may be used as an economical scouting tool to investigate the con-
ditions for purifying a protein before using a standard UNO column. The polishing
columns are constructed with biocompatible PEEK and polyethylene frits to main-
tain biological activity to insure biological activity of the sample.
The UNO Separation Medium
Each column contains a “Continuous Bed” support derivatized with either
strongly basic (-N + (CH
3
)
3
) or strongly acidic -SO
3
- sulfonic groups. The novel sup-
port produces excellent resolution of biomolecules at high flow rates and with low
back-pressures. The hydrophilic support demonstrates extremely low non-specific
binding of biomolecules accompanied by high recovery of biological activity.
Stability of the UNO Support
The columns are stable over the pH range 2–12, allowing for easy cleaning and
regeneration. The support is compatible with aqueous solutions of guanidine-HCl and
urea. Detergents and organic solvents such as 100% methanol, 100% ethanol, 100%
isopropanol, and 30% acetonitrile may also be used.
Connection to Chromatography Systems
Each UNO column is supplied with a fittings kit containing two pieces of 0.02
inch ID (1/16 inch OD) Tefzel tubing and two 1/4 x 28 flangeless fittings. Assemble
one 1/4 x 28 fitting onto one end of the 0.02” tubing and insert each fitting into the
top and bottom of the column. For connection to your chromatography system, attach
the appropriate fitting onto the bare end of the Tefzel tubing.
Note: Bio-Rad Laboratories does not recommend nor warranty the use of the
UNO columns with solvent delivery systems containing stainless steel parts
when used with corrosive eluents containing e.g. halide salts. We recommend the
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