Bio-Rad Model 2110 Fraction Collector - Manual (Page 18)

Time Mode
The fraction collector collects fractions at intervals of .01 minutes, for
times per tube in the range of .05 to 9.99 minutes, and at.10 minute inter-
vals for times per tube in the range of 10.0 to 99.9 minutes.
1. Press the Run/Stop key until the LED is lit.
2. Press the Time/Drop key until the LED is lit.
3. Select the time interval per tube by pressing the
keys. The LED multi-display will display
the time interval selected.
4. Insure that the carousel is in the desired position.
Press the key to begin the program. The
LED will be lit.
5. The fraction collector will reset the display to ‘0’,
and begin counting time up to the set time inter-
val, and then advance.
Note: When the Model 2110 Fraction Collector reaches the preset
time interval in a time mode collection scheme, it will collect one
extra drop prior to advancing. This minimizes the chance of a drop
falling between tubes during carousel advance. If the delay between
drops is greater than 5% of the preset time interval, the collector will
advance to the next fraction instead of waiting for one extra drop.
6.3 Operation without Direct Connection
to a System
The Model 2110 can be used in stand-alone mode with any system.
Simply set up the collector in time mode or drop mode and press “start”
at the initiation of the method.
Alternatively, a light-weight gravity feed column (such as 2 ml
Poly-Prep ® columns, Econo-Pac ® columns, and small Econo-Column ®
chromatography columns) can be connected directly to the Model 2110
Fraction Collector, avoiding the need for interconnecting tubing. A
two-way stopcock is used to make the connection and control eluant flow.
ml
min
ml/min
%
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