Bio-Rad Bio-Plex Pro™ Human Isotyping Assays - Manual

IMPORTANT! Pay close attention to vortexing, shaking, and incubation instructions.
Deviation from the protocol may result in low assay signal and assay variability.
For use with
Instruction Manual #
Bio-Plex Pro Human Isotyping Assays 10028370
This guide can be used to prepare and run a full 1 x 96-well assay plate.
For more information on a given step, refer to the complete instruction
manual. New users can download the manual, which includes detailed
instructions and a list of kit components, at www.bio-rad.com/bio-plex.
Initial Preparation
1. Plan the plate layout.
2. Start up/warm up the Bio-Plex ® system (30 min).
n
Bring the 10x wash buffer, assay buffer, and isotyping diluent to room
temperature (RT). Keep other items on ice until needed
n
Begin to thaw frozen samples
3. Prime wash station for flat bottom plate or set vacuum manifold to
–1 to –3" Hg for filter plate.
4. Calibrate the Bio-Plex system by following the prompts within the
Bio-Plex Manager ™ software. This can be done now or during an
assay incubation step.
5. Prepare 1x wash buffer. Mix 10x stock by inversion to ensure all salts are
in solution. Then dilute 1 part 10x wash buffer with 9 parts dH
2
0.
Bio-Plex Pro ™ Assays
Quick Guide 6