Bio-Rad Apoptosis Assays - Manual (Page 2)

B. Dilution of Standard (1:3 Serial Dilution)
1. Label 8 polypropylene tubes S1 through S8.
2. Transfer the reconstituted standard into the tube labeled “S1.”
3. Add the appropriate amount of the standard diluent into the labeled
tubes according to the table below (this will be sufficient for duplicate
standard curves and blanks).
4. Prepare working standards (S2–S8) by serial dilution. Transfer the
appropriate volume of standard into each of the labeled tubes with
standard diluent as outlined above.
5. Vortex each standard at a medium setting before proceeding with the next
serial dilution. Change pipet tip at each dilution step.
C. Sample Preparation
1. Refer to instruction manual #10033631 for detailed sample preparation.
Note: Pay close attention to cell lysis, homogenization, and fractionation
protocols in the manual. Analytes of interest may be localized to the
cytosolic fraction or to the nuclear and mitochondrial fraction.
2. Prepare sample dilutions in 0.5 ml or 1.0 ml polypropylene tubes as
required for the assay.
Bio-Plex Pro Assay Quick Guide
Standard Volume of Standard Diluent, µl Volume of Standard, µl
S2 100 50 of S1
S3 100 50 of S2
S4 100 50 of S3
S5 100 50 of S4
S6 100 50 of S5
S7 100 50 of S6
S8 100 50 of S7
Blank 100 —