Bio-Rad Bio-Plex Pro™ TGF-β Assays - Manual (Page 4)

13. Cover and incubate at 850 ± 50 rpm, as in Step 5, for 30 min at RT.
14. Wash the plate three times with 100 µl wash buffer.
15. Resuspend beads in 125 µl assay buffer. Cover and shake at
850 ± 50 rpm, as in Step 5, for 30 sec.
16. Remove the sealing tape and read plate using the settings below.
Bio-Plex Pro Assay Quick Guide 5
Instrument RP1 (PMT) DD Gates Bead Events
Bio-Plex 100, 200* Low 5,000 (low), 25,000 (high) 50
Bio-Plex 3D* Standard Select MagPlex beads 50
Bio-Plex ® MAGPIX ™ * N/A, use default instrument settings Default
* Or similar Luminex-based system.
MagPlex is a trademark of Luminex Corporation.
The Bio-Plex suspension array system includes fluorescently labeled microspheres and
instrumentation licensed to Bio-Rad Laboratories, Inc. by the Luminex Corporation.
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