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Bio-Rad Bio-Plex Pro™ TGF-β Assays User Manual

Page 4

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13. Cover and incubate at 850 ± 50 rpm, as in Step 5, for 30 min at RT.

14. Wash the plate three times with 100 µl wash buffer.

15. Resuspend beads in 125 µl assay buffer. Cover and shake at

850 ± 50 rpm, as in Step 5, for 30 sec.

16. Remove the sealing tape and read plate using the settings below.

Bio-Plex Pro Assay Quick Guide 5

Instrument

RP1 (PMT)

DD Gates

Bead Events

Bio-Plex 100, 200*

Low

5,000 (low), 25,000 (high)

50

Bio-Plex 3D*

Standard

Select MagPlex beads

50

Bio-Plex

®

MAGPIX

* N/A, use default instrument settings

Default

* Or similar Luminex-based system.

MagPlex is a trademark of Luminex Corporation.
The Bio-Plex suspension array system includes fluorescently labeled microspheres and
instrumentation licensed to Bio-Rad Laboratories, Inc. by the Luminex Corporation.

Life Science

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Sig 1212

10024986 Rev A

Bio-Rad

Laboratories, Inc.

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