Bio-Rad Bio-Plex Pro™ Rat Cytokine, Chemokine, and Growth Factor Assays - Manual

This guide can be used to prepare and run a full 1 x 96-well assay plate.
Refer to the complete instruction manual for more information on a given
step. New users can download the manual, which includes detailed
instructions and a list of kit components, at www.bio-rad.com/bio-plex.
Initial Preparation
1. Plan the plate layout.
2. Start up/warm up the Bio-Plex ® system (30 min).
n
Bring assay buffer, wash buffer, and sample diluent to room
temperature (RT). Keep other items on ice until needed
n
Begin to thaw frozen samples
3. Prime wash station for flat bottom plate or set vacuum manifold to
–1 to –3" Hg for filter plate.
4. Calibrate the Bio-Plex system by following the prompts within
Bio-Plex Manager ™ software. This can be done now or during an
assay incubation step.
5. Reconstitute a single vial of standards in 500 µl of a diluent similar to the
final sample type or matrix. Vortex for 5 sec and incubate on ice for 30 min.
Bio-Plex Pro ™ Assays
Quick Guide 4
IMPORTANT! Pay close attention to vortexing, shaking, and incubation instructions.
Deviation from the protocol may result in low assay signal and assay variability.
For use with
Instruction Manual #
Human, Mouse, and Rat Cytokine Assays 10014905
Sample Type Diluent for Standards Add BSA
Serum and plasma Standard diluent None
Culture media, with serum Culture media None
Culture media, serum-free Culture media To 0.5% final (w/v)