Bio-Rad Bio-Plex Pro™ Human Cancer Biomarker Assays - Manual

IMPORTANT! Pay close attention to vortexing, shaking, and incubation instructions.
Deviation from the protocol may result in low assay signal and assay variability.
For Use with*
Instruction Manual #
Bio-Plex Pro Human Cancer Biomarker Panel 1 and Panel 2 10022186
Bio-Plex Pro Human Th17 Cytokine Panel 10023381
* Includes premixed multiplex panels, singleplex sets, custom x-Plex
™ assays, and Express assays.
This guide can be used to prepare and run a full 1 x 96-well assay plate.
For more information on a given step, refer to the corresponding section
of the complete instruction manual. New users can download the manual,
which includes detailed instructions and a list of kit components, at
www.bio-rad.com/bio-plex.
Initial Preparation
1. Plan the plate layout. (Section 1 of the complete instruction manual)
2. Start up/warm up the Bio-Plex ® system (30 min). (Section 2)
n
Meanwhile, bring buffers and diluents to room temperature (RT)
n
Begin to thaw frozen samples
3. Prime wash station or set vacuum manifold to –1 to –3" Hg. (Section 3)
4. Calibrate the Bio-Plex system by following the prompts within the
Bio-Plex Manager ™ software. This can be done now or during an
assay incubation step. (Section 2)
Bio-Plex Pro ™ Assays
Quick Guide 1