Bio-Rad Zeta-Probe Membranes - Manual (Page 16)

10% BLOTTO g/100 ml
Nonfat powdered milk 10
0.2% sodium azide 0.2
Store at 4°C
20% SDS MW g/L
20% sodium dodecyl sulfate 288.38 200
Heat to 65°C to get into solution
1 M Na
2
HPO
4
, pH 7.2 MW g/L
1 M Na
2
HPO
4
•7 H
2
O 268.07 268.07
Add 4 ml 85% H
3
PO
4
[1 M in Na + , see Reference 4]
50% Dextran Sulfate MW g/100 ml
50% dextran sulfate 500,000 50
0.2% sodium azide 65.01 0.2
Store at 4°C
50% Formamide g/100 ml
50% formamide 50
Store at 4°C. Immediately before use, deionize the required volume by stirring
gently for 1 hr with 1 g mixed-bed ion exchange resin (AG
® 501-X8(D) resin,
catalog #142-6425) per 10 ml of formamide. Filter through coarse filter paper.
6 M Glyoxal (Deionized)
Deionize 6 M glyoxal by pouring over a small mixed-bed resin column (AG 501-
X8 mixed bed ion exchange resin, catalog #142-6424). Store at –20°C in small
aliquots. Once an aliquot has been exposed to air, it cannot be reused.
27
Section 8
Appendix
20x TAE MW g/L
0.8 M Tris 121.1 96.9
0.4 M base sodium acetate 82.04 32.8
20 mM EDTA 372.2 7.45
pH to 7.4 with glacial acetic acid
5x TBE MW g/L
0.5 M boric acid 61.8 30.9
0.5 M Tris base 121.1 60.5
10 mM EDTA 372.2 3.73
20x SSC MW g/L
3 M NaCl 58.44 175.0
0.3 M trisodium citrate 294.1 88.2
20x SSPE MW g/L
3.6 M NaCl 58.44 210.0
0.2 M Na
2
HPO
4
•7 H
2
O 268.07 53.6
20 mM EDTA 372.2 7.44
TE
10 mM Tris-HCl, pH 8.0
1 mM EDTA, pH 8.0
100x Denhardt's Solution MW g/100 ml
2% bovine serum albumin 2
2% polyvinylpyrrolidone 360,000 2
2% Ficoll 400,000 2
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