Bio-Rad C/P Lift Membranes - Manual (Page 3)

Section 1
Introduction
C/P Lift membranes were developed for colony and
plaque screening. When handling the membranes,
always wear gloves or use forceps. After blotting, do not
allow wet membranes to come in contact with each
other. Contact may result in the transfer of blotted
nucleic acids from one membrane to the other.
Section 2
Colony Screening
2.1 Direct Lifts
1. Plate cells containing recombinant plasmids onto the
appropriate selective agar. Incubate plated cells until
the colonies are 1-2 mm in diameter.
2. Place a circle of C/P Lift membrane onto the surface
of the plate as follows. Hold the membrane at
opposite edges with forceps, bending it slightly into
a ‘U’-shape, and then lower the membrane until the
fold makes contact with the center of the plate.
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