Bio-Rad CHEF Genomic DNA Plug Kits - Manual (Page 2)

Table of Contents
Section 1 Introduction......................................................1
1.1 Kit Components ..........................................................2
Section 2 Preparation of Agarose Embedded
Mammalian DNA ............................................3
Section 3 Preparation of Agarose Embedded
Bacterial DNA ..................................................5
Section 4 Preparation of Agarose
Embedded Yeast DNA ....................................8
Section 5 Restriction Enzyme Digestion of Plugs ........11
Section 6 References ......................................................12
Section 7 Additional Reagents for Pulsed Field
Electrophoresis ..............................................13
Section 8 Instruments for Pulsed Field
Electrophoresis ..............................................13
Section 9 Appendix ........................................................14
9.1 Solutions ....................................................................14
9.2 Agarose Concentrations ............................................15
9.3 Hemocytometer Usage ..............................................16
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