Bio-Rad Model 422 Electro-Eluter - Manual (Page 3)

Table of Contents
Section 1 Introduction..................................................................................................1
1.1 Specifications .............................................................................................................1
Section 2 Equipment and Reagents............................................................................2
2.1 Equipment ..................................................................................................................2
2.2
Modular Mini Electrophoresis System .....................................................................2
Section 3 Safety Instructions.......................................................................................3
Section 4 Assembly .......................................................................................................4
Section 5 Visualizing Proteins in SDS-Polyacrylamide Gels ...................................5
5.1 Visualizing Proteins Stained with Coomassie Blue R-250 ......................................5
5.2 Visualizing Proteins Using Copper Chloride ...........................................................6
5.3 Visualizing Proteins Using Autoradiography ..........................................................6
Section 6 Protein Elution ............................................................................................6
6.1 Elution from SDS-PAGE Gels .................................................................................6
6.2 Elution of Fixed and Stained Proteins ......................................................................7
6.3 Concentration of Eluted Proteins Using Volatile Buffers.........................................7
6.4 Elution of Proteins from Native Gels .......................................................................7
6.5 Removal of SDS from Samples Subsequent to Elution............................................8
Section 7 Visualizing DNA.......................................................................................... 9
7.1 Visualizing DNA in Agarose Gels ............................................................................9
7.2 Visualizing DNA in Acrylamide Gels.......................................................................9
Section 8 DNA Elution .................................................................................................9
8.1 Elution from Gel ........................................................................................................9
8.2 DNA Purification.....................................................................................................10
Section 9 Recipies .......................................................................................................11
Section 10 Troubleshooting Guide..............................................................................11
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